2024年4月25日（木）、オンラインにて、「LINK-J & UCサンディエゴ ジョイントウェビナーシリーズ 第14回 with 大阪大学国際医工情報センター「培養組織に対する物理刺激による機能化 ～Session 5 細胞・基質の調製を通した精密な細胞モデリング～」」を開催いたしました。今回は、UCサンディエゴと大阪大学国際医工情報センター（MEIセンター）が協力し、両校の研究者が「細胞・基質の調製を通した精密な細胞モデリング」に関して最新の研究成果を発表しました。
【登壇者】
Dr. Andrew McCulloch, Distinguished Professor of Bioengineering and Medicine; Director, Institute of Engineering in Medicine, UC San Diego
Dr. Masahiro Kino-oka, Professor, Department of Biotechnology, Graduate School of Engineering, Osaka University
Dr. Brian Aguado, Assistant Professor of Bioengineering, Shu Chien-Gene Lay Department of Bioengineering at the UC San Diego Jacobs School of Engineering
Dr. Mee-Hae Kim, Associate Professor at the Department of Biotechnology, Osaka University Graduate School of Engineering
高橋 俊一（LINK-J事務局長）
和賀 三和子氏（UC San Diego国際イノベーションアウトリーチシニアディレクター）
冒頭に紀ノ岡先生とAndrew McCulloch博士よりご挨拶および講演者の紹介を頂き、パネルディスカッションではモデレーターとして質疑応答を行いました。ご講演のアブストラクトは以下のとおりです。
Dr. Brian's talk
"Determining sex differences in cardiovascular disease using biomaterials"
Cardiovascular disease is the leading cause of death in both men and women, yet our mechanistic knowledge of the sex-specific molecular and cellular mechanisms that guide cardiovascular disease progression, particularly in women, remain poorly characterized. Studies evaluating disease mechanisms rarely state the sex of cells used for in vitro studies or are performed primarily in male animal models, causing our gap in knowledge. My laboratory uses precision biomaterials as in vitro and in vivo tools to dissect mechanisms that contribute to sexual dimorphisms in cardiovascular diseases, specifically aortic valve stenosis.
In my talk, I will discuss how we have used hydrogel biomaterials as engineered valve matrix mimics to explore sex dimorphisms in valvular interstitial cell phenotypes in vitro and describe sex-specific molecular mechanisms that may drive dimorphisms in aortic valve stenosis. Our work seeks to leverage biomaterial technologies to understand sex dimorphisms in health and disease, with the long-term goal of achieving sex and gender equity in cardiovascular disease treatments and outcomes.
Dr. Mee-Hae's talk
"Development of Culture Strategy for the Synchronous Differentiation of Human Induced Pluripotent Stem Cells"
To industrialize induced pluripotent stem cell (iPSC)-derived therapies, it is necessary to demonstrate the safety and efficacy of these therapies and develop robust and controllable bioprocesses to produce iPSC-derived functional cells. However, developing culture strategies for bioprocesses remains challenging because there is no efficient way to synchronize their differentiation stages.
This webinar will provide a novel culture strategy to improve the differentiation stability of iPSCs and their efficiency in giving rise to functional cells. First, we will briefly introduce the impact of mechanical forces within the cellular environment and identify key cell state determinants in iPSC expansion and differentiation. Specifically, we will focus on the manner in which cell behavior triggers mechano-chemical transduction signaling pathways in relation to the initial cell fate decision. We will then introduce the effect of the botulinum hemagglutinin (HA) on cell synchronization to achieve homogeneous population of differentiated cells. Temporal disruption of cell‐cell interactions by HA suppresses spatial heterogeneity of cells within the culture and synchronize mechanical memory via YAP localization. The homogeneous population of cells with the synchronized mechanical memory have higher capability to differentiate into functional cells than unsynchronized iPSCs. Finally, we will discuss the design principles of synchronous differentiation strategy for iPSCs based on the concept of Waddington's epigenetic landscape and it potential application to an existing culture technologies.
当日の模様は、LINK-JのYouTubeチャンネルでアーカイブ動画として公開しておりますので、是非ご覧ください。
講演後はQ＆Aセッションを実施しました。紀ノ岡先生とAndrew McCulloch博士がモデレーターとなり、視聴者からの質問を受け付け、様々な議論が交わされました。
ご参加いただいた皆様ありがとうございました。今後もUC San Diegoとの共催ウェビナーを継続的に開催する予定です。
皆様ぜひご参加ください。
